| 石丽娟,朱建思,王成昆,周建国,董琳,雷战平.外源性p16基因导入对人原发性肝癌细胞迁移能力和VEGF及nm23表达的影响.[J].中南医学科学杂志.,2006,(2):195-199. |
| 外源性p16基因导入对人原发性肝癌细胞迁移能力和VEGF及nm23表达的影响 |
| The Effect of Exogenous p16 Expression on Migration Ability, VEGF and nm23 Expression of Human Hepatocellular Carcinoma |
| 投稿时间：2006-02-16 |
| DOI： |
| 中文关键词: 肝肿瘤 迁移 |
| 英文关键词:p16 VEGF nm23 |
| 基金项目: |
| 石丽娟 朱建思 王成昆 周建国 董琳 雷战平 |
| 南华大学肿瘤研究所,湖南衡阳421001 |
| 摘要点击次数: 752 |
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| 中文摘要: |
| 目的 探讨外源性p16基因导入对人原发性肝癌细胞迁移能力和VEGF及nm23表达的影响。方法 细胞划痕法检测外源性p16基因导入对人肝癌细胞SMMC-7721迁移能力的影响；免疫细胞化学法分析外源性p16基因导入对VEGF表达的影响；Western blotting法分析外源性p16基因导入对nm23表达的影响。结果 外源性p16基因导入人肝癌细胞SMMC-7721后，转染组细胞迁移能力较空载体对照组和空白对照组SMMC-7721细胞明显下降。外源性p16基因导入人肝癌细胞SMMC-7721后，转染组VEGF表达较空载体对照组和空白对照组SMMC-7721细胞减弱。外源性p16基因导入人肝癌细胞SMMC-7721后，转染组nm23表达较空载体对照组和空白对照组SMMC-7721细胞增强。结论 外源性p16基因导入能降低人肝癌细胞SMMC-7721的侵袭和迁移能力。 |
| 英文摘要: |
| Objective To investigate the effect of exogenous p16 expression on migration ability,VEGF and nm23 expression of human hepatocellular carcinoma. Methods The migration abilities of SMMC-7721 cell were tested using a scratchwound model in vitro.Protein level of vascular endothelial growth factor(VEGF) was assessed by immunocytochemistry.Protein level of nm23 was assessed by Western blot.Results The migration abilities of SMMC-7721-p16 cells was obviously decreased compared with that of SMMC-7721-VECTOR and SMMC-7721 cells.Protein level of vascular endothelial growth factor(VEGF) of SMMC-7721-p16 cells was decreased compared with that of SMMC-7721-VECTOR and SMMC-7721 cells.Protein level of nm23 of SMMC-7721p16 cells was increased compared with that of SMMC-7721-VECTOR and SMMC-7721 cells.Conclusions Exogenous p16 expression can decrease the invasion and migration abilities of SMMC-7721 cells. |
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