彭军,肖晓岚,苏琦,向姝霖,董琳.二烯丙基三硫诱导人胃癌细胞凋亡的作用.[J].中南医学科学杂志.,2005,(4):453-456.
二烯丙基三硫诱导人胃癌细胞凋亡的作用
Apoptosis of Human Gastric Cancer Cell Line MGC - 803 Induced by Diallyl Trisulfide
  修订日期:2005-08-11
DOI:
中文关键词:  二烯丙基三硫  胃肿瘤  MGC-803细胞  凋亡
英文关键词:diallyl trisulfide,stomach tumor,MGC-803 cell line,apoptosis
基金项目:湖南省教育厅课题(03SSY3103),湖南省科研计划重大专项(04SK1004)
彭军  肖晓岚  苏琦  向姝霖  董琳
[1]株洲市一医院,湖南株洲412000 [2]南华大学肿瘤研究所,湖南衡阳421001
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中文摘要:
      目的 探讨二烯丙基三硫(diallyl trisulfede,DAlS)诱导人胃癌MGC-803细胞凋亡的作用。方法 采用倒置显微镜、光学显微镜、透射电镜分别观察DATS诱导人胃癌MGC-803细胞凋亡的形态学改变以及用流式细胞术观察亚G1峰。结果 MGC-803细胞经DATS处理24h后,光镜可见细胞胞浆浓缩、核固缩深染等早期凋亡细胞;电镜可见到不同时期凋亡细胞;流式细胞术检测有亚G1峰的出现。结论 DATS具有诱导胃癌MGC-803细胞凋亡的作用。
英文摘要:
      Objective To investigate whether DATS induces apoptosis in MGC-803 cells.Methods Cell apoptosis was inspected by invert microscope,light microscope,electron microscope and flow cytometry.Results After 24 hours of exposure to DATS,partial cells presented characteristic morphological changes of apoptosis,including cell shrinkage,nuclear condensation.Flow cytometry analysis showed that treating MGC-803 cells with DATS significantly increased the percentage of apoptosis cells.Conclusion Diallyl trisulfide could significantly induce apoptosis in MGC-803 cells.
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