| 周宇清,曹仁贤,文芳,谢翠松.软脂酸对胰岛细胞凋亡作用的观察.[J].中南医学科学杂志.,2005,(2):196-199. |
| 软脂酸对胰岛细胞凋亡作用的观察 |
| Apoptosis-inducing Effect of Palmitic Acids on Rat Pancreatic Islet Cells |
| 修订日期：2005-01-28 |
| DOI： |
| 中文关键词: 软脂酸 胰岛细胞 凋亡 |
| 英文关键词:palmitic acid,pancreatic islet cells,apoptosis |
| 基金项目:湖南省教育厅课题 (9615 9) |
| 周宇清 曹仁贤 文芳 谢翠松 |
| 南华大学第一附属医院,湖南衡阳421001 |
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| 中文摘要: |
| 目的通过观察软脂酸对体外培养的原代胰岛细胞的凋亡作用，探讨游离脂肪酸在糖尿病发病中的作用。方法体外培养Wistar大鼠胰岛细胞，用不同浓度软脂酸(分别为0，0．125，0．25，0．5mmol／L)共同孵育48h，用Tunel法计算胰岛细胞凋亡率。AO／EB染色观察细胞形态变化。结果0．125，0．25，0．5mmol／L的软脂酸均有抑制高糖刺激下胰岛细胞的胰岛素分泌，促进胰岛细胞凋亡的作用。结论软脂酸可抑制胰岛细胞的分泌功能，促进胰岛细胞凋亡，其凋亡程度与软脂酸的浓度有关。 |
| 英文摘要: |
| Objective To investigate the effect of a chronic exposure to high level palmitic acid on islet cell apoptosis, thereby to understand the role of palmitic acid in the pathogenesis of type 2 diabetes mellitus. Methods Wistar rat pancreatic islet cells were isolated and cultured in monolayer in vitro with or without palmitic acid (0.125,0.25 ,0.5 mmol/L),and the insulin concentrations in culture medium were determined by radio immunological methods. The percentage of apoptotic cells was assessed by Tunel.Morphological observation with a fluorescence microscope was conducted after double staining of the cells with AO/EB. Result Palmitic acid with different concentrations(0.125,0.25 ,0.5 mmol/L )all have the effects of inhibiting insulin secretion of pancreatic islet cells and inducing islet cells apoptosis. Conclusion Palmitic acid is capable of inducing islet cells apoptosis in dose-dependent manner |
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