| 肖晓岚,向姝霖,唐国华,周秀田,谢娜,葛玲,苏琦.二烯丙基三硫对MGC-803细胞凋亡的影响.[J].中南医学科学杂志.,2005,(2):167-170. |
| 二烯丙基三硫对MGC-803细胞凋亡的影响 |
| The Effect of DATS Inducing Apoptosis of MGC-803 |
| 修订日期：2005-03-03 |
| DOI： |
| 中文关键词: 二烯丙基三硫 胃肿瘤 凋亡 活性氧 |
| 英文关键词:diallyl trisulfide,stomach tumor,apoptosis,reactive oxygen species |
| 基金项目:湖南省教育厅课题 (No 0 3SSY3 10 3 )，湖南省科研计划重大专项 (No 0 4SK10 0 4) |
| 肖晓岚 向姝霖 唐国华 周秀田 谢娜 葛玲 苏琦 |
| 南华大学肿瘤研究所,湖南衡阳421001 |
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| 中文摘要: |
| 目的探讨二烯丙基三硫(dially disulfide，DATS)诱导人胃癌MGC-803细胞凋亡及作用机制。方法光学显微镜观察凋亡形态，流式细胞术检测凋亡及细胞内活性氧水平。结果12mg／LDATS作用于胃癌MGC-803细胞24小时后，在光学显微镜下出现了典型的凋亡细胞形态学改变，流式细胞仪检测结果提示，活性氧水平升高，出现明显的亚G1峰。用抗氧化剂NAC预处理细胞，能抑制细胞内活性氧水平的升高及DATS诱导的凋亡。结论DATS诱导胃癌细胞凋亡可能与细胞内活性氧产生增加有关。 |
| 英文摘要: |
| Objective To investigate DATS inducing MGC-803 cell apoptosis and its possible mechanism. Methods The apoptotic morphological change was observed under the light microscope. Intracellular reactive oxygen species (ROS) level and the rate of apoptosis were detected by the flow cytometry. Results After MGC-803 cells were treated with 12 mg/L DATS for 24 hours, the typical apoptotic morphological changes were observed under the light microscope, flow cytometry analysis showed that intracellular ROS level and the percentage of apoptosis cell were significantly increased after treating MGC-803 with DATS. Pretreatmentof N-acetyl-l-cystein, a thiol-containing antioxidant, effectively attenuates DATS-induced increasing of intracellular ROS and the consequent apoptotic of MGC803. Conclusion These results suggested that the cellular generation of ROS may play a role in initiating DATS-mediated apoptosis of MGC-803 cells. |
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