| 万志刚,肖建华,曾桥,杨秋林,张愉快,杨胜.日本血吸虫新基因BBC1的获得和分析.[J].中南医学科学杂志.,2004,(3):275-278. |
| 日本血吸虫新基因BBC1的获得和分析 |
| Acquiring and Sequence Analysis of the Breast Basic Conserved ( BBC1) Gene of Schistosoma Japonicum |
| 修订日期：2004-03-28 |
| DOI： |
| 中文关键词: 日本血吸虫 cDNA文库 步移法 生物信息学 |
| 英文关键词:Schistosoma japonicum,cDNA library,Primer-walking,Bioinformatics |
| 基金项目:湖南省卫生厅重点项目 (No .2 0 0 1-Z0 5 ) |
| 万志刚 肖建华 曾桥 杨秋林 张愉快 杨胜 |
| 南华大学病原生物学研究所,湖南衡阳421001 |
| 摘要点击次数: 724 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 从日本血吸虫(Schistosoma japonicum，Sj)成虫cDNA文库中获得并分析日本血吸虫新的蛋白基因，为日本血吸虫病的防治提供候选疫苗和药物靶点。方法 构建日本血吸虫成虫cDNA文库，挑取重组阳性克隆进行测序，对部分序列进行步移法测序获取全长cDNA，并进行生物信息学分析和登录。结果 获得了1个日本血吸虫新基因一BBC1基因(AY220747)，长623bp，编码184个氨基酸，其编码蛋白的理论分子量为60．8kDa，等电点为5．13。结论 步移法测序和生物信息学技术相结合有利于发现日本血吸虫新基因。 |
| 英文摘要: |
| Objective To acquire and analyze adult stage Schistosoma japonicum (Sj) new genes from an adult Schistosoma japonicum cDNA library and to search new vaccine candidates and drug targets for preventing, controlling and treating Schistosomasis. Methods A cDNA library was constructed from Shistosoma japonicum (Chinese strain) adult stage. Clones were selected randomly from the cDNA library and were sequenced. Novel genes were analyzed in GenBank databases by BLAST programs and other programs. Results BBC1 gene, 623 base pairs and coding for 184 amino acids, was acquired and was submitted to GenBank (AY220747). The molecular weight is 60.8 kDa and the theoretical pI is 5.13. Conclusion It is a feasible method to discover and acquire novel genes of Schistosoma japonicum by combination of Primer-walking sequencing and Bioinformatics technique. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|