| 张前军,祖旭宇,梁宋平,李敏.拖丝蛋白全基因亚克隆文库的构建和部分序列的测定.[J].中南医学科学杂志.,2004,(2):151-154. |
| 拖丝蛋白全基因亚克隆文库的构建和部分序列的测定 |
| Construction of Subclone Library of Dragline and the Determination of Its Partial Sequence |
| 修订日期：2003-12-27 |
| DOI： |
| 中文关键词: 亚克隆 拖丝蛋白 蜘蛛 |
| 英文关键词:subclone,dragline,spide |
| 基金项目: |
| 张前军 祖旭宇 梁宋平 李敏 |
| [1]南华大学生命科学学院,湖南衡阳421001 [2]湖南师范大学生命科学学院,湖南衡阳421001 |
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| 中文摘要: |
| 目的 为获得蜘蛛拖丝蛋白全基因。方法 以斑点杂交、Southem印迹结果证实了的蜘蛛拖丝蛋白全基因的克隆，构建其亚克隆文库，采用鸟枪法测序的策略，选取以HinfI为酶切工具，对Scos—DS1的DNA做大量酶切，以pUC19为载体，取插入片段大小为500bp左右的阳性重组子100个构建拖丝蛋白基因的亚克隆文库。结果 成功地构建了蛛丝蛋白基因Scos—DS1由HinfI酶切片段连接而成的亚克隆文库，得到了其部分序列。结论 亚克隆方法可以得到拖丝蛋白基因全序列。 |
| 英文摘要: |
| Objective To obtain the whole length gene of dragline.Methods The Scos-DS1 DNA was digested with Hinf I and the subclone library of dragline was constructed using pUC19 as the vecter .After screening the library, 100 clones with insert around 500 bp in size were taken to determine the sequence.Results The subclone library of dragline gene connected by enzymolytic fragment of HinfI was succesfully constructed and its partial sequences were gained. Conclusion The whole length gene of dragline can be gained by using subclone method. |
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