| 肖华斌,包杰,周江南.Rb反义寡核苷酸对人骨肉瘤细胞凋亡及化疗药敏的影响.[J].中南医学科学杂志.,2004,(1):54-57. |
| Rb反义寡核苷酸对人骨肉瘤细胞凋亡及化疗药敏的影响 |
| The Effects of Rb AS-ODN on Apoptosis and Drug Sensitivity of Osteogeinc Sarcoma |
| 修订日期：2003-11-08 |
| DOI： |
| 中文关键词: 骨肉瘤 寡聚核苷酸 Rb基因 凋亡 药物敏感性 |
| 英文关键词:osteogeinc sarcoma,oligonucleotides,Rb gene,apoptosis,chemosensitivity |
| 基金项目: |
| 肖华斌 包杰 周江南 |
| [1]衡阳市中心医院,湖南衡阳421001 [2]中南大学湘雅医院骨外科,湖南衡阳421001 |
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| 中文摘要: |
| 目的:观察Rb反义寡核苷酸(AS—ODN)对人成骨肉瘤(MG-63)细胞凋亡及化疗药物敏感性的影响。方法:人工合成RbAS—ODN、S—ODN(正义寡核苷酸)经脂质体(Lip)包裹转染MG-63细胞。采用Westem Blot检测转染前后Rb蛋白表达状况，流式细胞术测定MG-63细胞转染前后阿霉素(Adriamycin，ADM)诱导细胞凋亡程度，MTT法观察化疗药敏的变化。结果:经Rb AS—ODN处理的MG-63细胞Rb蛋白表达量明显下降，RbAS—ODN处理的MG-63细胞加ADM作用后与对照组比较，细胞凋亡率明显下降，化疗药物敏感性降低。结论:RbAS—ODN阳离子脂质体转染具有抑制化疗药诱导骨肉癌MG-63细胞凋亡的作用及降低化疗药物敏威性作用。 |
| 英文摘要: |
| Aim To investigate the effects of Rb AS-ODN on apoptosis and drug sensitivity of osteosarcoma cell. Methods MG-63 cells were transfected with Rb AS-ODN by cationic liposome, S-ODN and LP in vitro.The expression of pRb treated with or without AS-ODN ,S-ODNand LP in MG-63 cells was observed by Western blot. The Flow cytometer(FCM) was used to examine apoptosis induced by ADM in MG-63 cells. The MTT assay was used to measure chemotherapeutic agent-sensitivity of MG-63 cells treated with ADM. Results The level of pRb expression in the MG-63 osteosarcoma line transfected with Rb AS-ODN obviously reduced compared with that of controls ,which were treated with S-ODN,LP and non-transfected(P<0.05). The percentage of the apoptotic cells and drug-sensitivity of MG-63 cells treated with Rb AS-ODN and ADM decreased compared with that of the controls(P<0.05). Conclusion The transfection of Rb AS-ODN delivered by cationic liposome could inhibit apoptosis induced with ADM in MG-63 cells and decrease drug-sensitivity of MG-63 cells to ADM. |
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