| 万炜,何洁,陈熙,李学军,邓盘月,罗学港.大鼠脊髓全横断后TrkA和NGF在运动皮质的免疫组化研究.[J].中南医学科学杂志.,2003,(3):257-259,274. |
| 大鼠脊髓全横断后TrkA和NGF在运动皮质的免疫组化研究 |
| The Immunohistochemical Study of TrkA and NGF in Adult Rat Motor Cortex Following Spinal Cord Complete Transection |
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| DOI： |
| 中文关键词: 大鼠 脊髓全横断 TrkA NGF 运动皮质 免疫组化 神经营养因子 脊髓损伤 |
| 英文关键词:spinal cord complete transection,TrkA,NGF,motor cortex |
| 基金项目:国家重点基础研究规划“脑功能和脑重大疾病的基础研究”(G19990 5 40 0 9)项目资助 |
| 万炜 何洁 陈熙 李学军 邓盘月 罗学港 |
| [1]南华大学解剖学教研室，湖南衡阳42100l [2]南华大学病理学教研室 [3]中南大学湘雅医院 [4]中南大学神经生物学研究室 |
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| 中文摘要: |
| 目的 探讨脊髓全横断后神经营养因子及相应受体在运动皮质内的分布规律，为临床应用神经生长因子治疗脊髓损伤提供实验依据。方法 用免疫组化方法和图像分析技术检测脊髓全横断后不同时相运动皮质内，神经生长因子(NGF)及其高亲和性受体TrkA的表达和分布。结果 各组运动皮质中TrkA和NGF阳性神经元主要分布在第5层；脊髓全横断3d后，运动皮质内TrkA表达上调，损伤后7d到达高峰，21d回到正常水平；NGF表达在损伤后7d上调，峰值出现在损伤后7d，21d回到正常水平。结论 NGF表达增高迟于TrkA，提示在损伤早期给予外源性NGF可能有利于受损的皮质脊髓束神经元的存活与再生． |
| 英文摘要: |
| Objective To study the pattern of the distribution of neurotrophin receptors tyrosine kinase A(TrkA) and nerve growth factor (NGF) in adult rat motor cortex following complete spinal cord transection (SCT). Methods With immunohistochemistry and quantitative analysis, the expression and distribution of neurotrophin receptors TrkA and NGF were detected in motor cortex following complete SCT. Results TrkA and NGF immunoreactive positive cells are mainly localized in motor cortex layer V in both control and experimental groups. The expression of TrkA in adult rat motor cortex was up-regulated after transection in day 3 and reached the peak in day 7; While the expression of NGF in motor cortex was up-regulated and reached the peak in day 7 after transection. Conclusion The up-regulation of NGF occurred earlier than that of NGF, the results suggest that administration of exogenous NGF may be beneficial for the survival and regeneration of the injured corticospinal neurons in the early period after injury. |
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