| 张蒙夏,唐圣松,谢富康.成年SD鼠骨髓基质干细胞培养及向神经元样细胞定向分化的研究.[J].中南医学科学杂志.,2003,(3):254-256. |
| 成年SD鼠骨髓基质干细胞培养及向神经元样细胞定向分化的研究 |
| Culture and Differentiation of Mesenchymal Stem Cells From Adult SD Rat Into Neuron-Like Cells |
| 修订日期：2003-03-21 |
| DOI： |
| 中文关键词: 成年SD鼠 骨髓基质干细胞 神经元 细胞定向分化 全反式维甲酸 细胞培养 |
| 英文关键词:mesenchymal stem cell,neuron,RA,differentiation |
| 基金项目:国家自然科学基金资助项目 ( 3 9970 2 3 7) |
| 张蒙夏 唐圣松 谢富康 |
| [1]南华大学组胚教研室，湖南衡阳42100l [2]南华大学药理学教研室 [3]广州中山大学中山医学院组胚教研室 |
| 摘要点击次数: 864 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨体外培养、扩增骨髓基质干细胞的方法，诱导骨髓基质干细胞向神经元样细胞定向分化。方法 采用全骨髓法培养成年SD大鼠股骨骨髓基质干细胞。传至第5代，用全反式维甲酸诱导分化，于诱导后第5小时行神经细胞特异性抗原MAP-2和胶质细胞特异性抗原GFAP鉴定。结果 成年鼠骨髓基质干细胞经全反式维甲酸诱导30min后可见神经元样细胞出现，经MAP-2免疫组化鉴定阳性。结论 (1)骨髓基质干细胞在体外扩增迅速，纯化需时短。(2)骨髓基质干细胞在全反式维甲酸诱导下向神经元样细胞分化。 |
| 英文摘要: |
| Objective To study the methods of culture and amplification of rat mesenchymal stem cells( MSCs) in vitro,and to induce MSC differentiating into the neuron-like cells. Methods The MSCs were isolated primarily from bone marrow of adult rat, and purified by passage culture. The 5th passage of MSCs were induced by all-trans-retinoic acid (RA) and immunocytochemical staining with anti-MAP-2, and GFAP were also performed on cells after five hours induction. Results Neuron-like cells appeared among MSCs after MSCs treated by RA. Neuron-like cells all were showed MAP-2 positive and GFAP negative. Conclusion MSC can be amplificated and purified in short time, be induced into neuronal-like cell by RA. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |