| 唐运莲,冬毕华,甘润良,曾庆彪,蒋日成.人胃癌及胃间质细胞肿瘤Epstein-Barr病毒的检测.[J].中南医学科学杂志.,2002,(1):11-13,18. |
| 人胃癌及胃间质细胞肿瘤Epstein-Barr病毒的检测 |
| Detection of Epstein-Barr Virus in Human Gastric Carcinomas and Gastric Stromal Tumours |
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| DOI： |
| 中文关键词: 胃肿瘤 腺癌 间质细胞肿瘤 EB病毒 检测 |
| 英文关键词:gastric neoplasm,adenocarcinoma,stromal tumours,Epstein-Barr virus(EBV) |
| 基金项目: |
| 唐运莲 冬毕华 甘润良 曾庆彪 蒋日成 |
南华大学肿瘤研究所 湖南衡阳421001
(唐运莲，冬毕华，甘润良，曾庆彪)
，南华大学肿瘤研究所 湖南衡阳421001(蒋日成)
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| 中文摘要: |
| 目的 探讨Epstein-Barr病毒感染对人类胃肿瘤发生，分化程度及淋巴结转移的影响。方法 取冰冻组织及石蜡切片提取DNA，用PCR方法扩增EBV-DNABamHI-W片段，阳性者提示有EBV感染，分析EBV阳性胃肿瘤的分化程度，淋巴细胞浸润和淋巴转移情况。结果 109例胃癌中共有18例检出EBV-DNA（阳性率为16.5%)，3例胃间质细胞肿瘤中有1例EBV-DNA阳性（阳性率为33.3%)。EBV阳性胃肿瘤分化程度低，淋巴结转移率低（r=-0.176,P=0.031)。富于淋巴细胞浸润，结论 EB病毒感染可能参与胃肿瘤的发生。Epstein-Barr病毒感染与淋巴结转移率呈负相关。 |
| 英文摘要: |
| Objective: To investigate the influence of Epstien-Barr virus infection on carcinogenesis, differentiation and lyphaden metastasis of gastric neoplasm .Methods: We extracted DNA genomes from frozen tissues and paraffin sections.Polymerase chain reaction(PCR) was used to amplify 129-bp fragment in BamHI-w region of EBV-DNA and the presence of EBV-DNA was evaluated.We analyzed the condition of differentiation, the infiltration of lymphocyte and lymphaden metastasis of EBV-positive gastric neoplasm. Results: EBV was observed in 18(16.5%) of 109 gastric carcinomas and in 1(33.3%) of 3 gastric stromal tumours .The rate of lymphaden metastasis in EBV-positive gastric carcinomas was lower than that in EBV-negative gastric carcinomas( r=-0.176, P=0.031).The EBV-positive gastric carcinomas was liable to be infiltrated by lymphocyte. Conclusions: EBV infection possibly affected the process of gastric carcinogenesis and negatively correlated with lymphaden metastasis of gastric carcinomas. |
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