| 郭玉,王芳宇,唐圣松,廖端芳.噬菌体随机肽库中与受损内皮细胞特异性结合短肽的筛选.[J].中南医学科学杂志.,2001,(6):535-538. |
| 噬菌体随机肽库中与受损内皮细胞特异性结合短肽的筛选 |
| Screening the Peptides Specifically Binding the Injured Endothelial Cell with Two Phage-Displayed Random Peptide Libraries |
| 修订日期：2001-09-06 |
| DOI： |
| 中文关键词: 噬菌体多肽文库,生物淘选法,内皮细胞 |
| 英文关键词:phage-displayed library,micropanning,endothelial cell |
| 基金项目:国家教育部资助课题，教技司 [2 0 0 0 ] 65号，湖南省自然基金及省卫生厅资助课题 |
| 郭玉 王芳宇 唐圣松 廖端芳 |
| 南华大学药物药理研究所，南华大学生物化学教研室，南华大学药物药理研究所，南华大学药物药理研究所 湖南衡阳421001，湖南衡阳421001 |
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| 中文摘要: |
| 目的 筛选与受损内皮细胞特异性结合的短肽。方法 以氧化型低密度脂蛋白 (oxidizedlowdensitylipoprotein ,ox -LDL)损伤牛胸主动脉内皮细胞 ,用丝状噬菌体 (FUSE5 )表面展示的随机 6肽和 15肽文库进行混合筛选 ,通过DNA序列测定确定其插入的氨基酸序列。结果 经过 3轮筛选 ,得到一些特异性结合的噬菌体克隆 ,随机挑选 10个克隆 ,进行DNA序列分析 ,其中 8个克隆含 6肽序列 ,2个克隆含 15肽序列。结论 用噬菌体技术能筛到与受损内皮细胞亲和力高、结合力强的特异性短肽 |
| 英文摘要: |
| Objective:To screen the peptides specifically binding the injured endothelial cell(EC) by means of both 6-mer and 15-mer peptides libraries displayed on the surface of filamentous bacteriophage(FUSE5).Methods:Cultured calf aortic endothelial cells were injured by incubating with oxidized low density lipoprotein(ox-LDL) for 4 hours.The binding phage to injured EC was obtained by three rounds of screening and the inserted amino acid sequences of displayed peptides was confirmed by DNA sequences.Results:We primarily isolated ten phage clones from two phage display libraries.Analysis of peptide shows that eight clones include 6-mer sequence and two clones contain 15-mer sequence.Conclusion:We obtained the peptides specifically binding with high avidity to injured EC by the phage-displayed approach. |
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