| 唐朝克,尹卫东.糖尿病新西兰兔模型的建立及并发糖尿病肾病的机理研究.[J].中南医学科学杂志.,2001,(5):439-442. |
| 糖尿病新西兰兔模型的建立及并发糖尿病肾病的机理研究 |
| Establishment of Diabetic Model in New Zealand White Rabbit and Studies on Mechanisms of Diabetic Nephropathy |
| 修订日期：2001-06-24 |
| DOI： |
| 中文关键词: 新西兰兔 糖尿病 一氧化氮 平均动脉压 血糖 肾病 |
| 英文关键词:New Zealand White rabbits,diabetes,nitric oxide,mean artery blood pressure,plasma glucose |
| 基金项目: |
| 唐朝克 尹卫东 |
南华大学生理学教研室 湖南衡阳421001
(唐朝克，尹卫东，徐刚，梁丽红，杨保堂)
，南华大学生理学教研室 湖南衡阳421001(文格波)
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| 中文摘要: |
| 目的：用新西兰兔建立一种新的糖尿病动物模型，探讨一氧化氮在糖尿病肾病发病机制中的作用。方法：采用高脂高糖饲料喂养新西兰兔。建立一种新的糖尿病动物模型。观察糖尿病新西兰兔平均动脉血压、血脂、胰岛素、肾脏病变和血浆、肾组织中的一氧化氮水平及一氧化氮合酶（NOS）活力变化。结果：糖尿病新西兰兔平均动脉血压增高（P＜0．05）；且出现大量蛋白尿（P＜0．05）；肌酐清除率（CCr）明显增高（P＜0．001）；血浆及肾组织中NO水平和NOS活力明显增高（P＜0．001）；血糖及胰岛素明显增高（P＜0．001）。结论：新西兰兔可用来建立糖尿病模型；NO是影响糖尿病新西兰兔肾小球高滤过的重要介质，肾脏NO系统异常与DN有关。 |
| 英文摘要: |
| Objective: To establish diabetic model in New Zealand rabbits and study the relationship between nitric oxide(NO) pathway and diabetic nephropathy.Methods:Two groups of New Zealand White rabbits received regular rabbit chow (the normal control) or high fat high sucrose diet for 4 months. The levels of plasma insulin and glucose were investigated. The level of renal NO and the activity of renal NO synthase (NOS) were measured besides morphological observation. Results:Glomerular lesions in test group involved mesangial cell proliferation trend of K-W node and fibrin cap and accumulation of mesangial matrix and thickness of glomerular membrane basement.There were still wall thickness and cavity stricture in renal resistant vasculature.Renal NO level and NOS activity were higher in test group than in control group(P<0.001). There was an elevation of the kidney/body weight in the early stage of the diabetes(P<0.05)as well as a great amount of proteinuria(P<0.05).The creatinine clearancerate(CCr) increased apparently(P<0.001). Conclusion:New Zealand White rabbits could be used to establish the model of diabetes; Nitric oxide might be the main inductive mediator of the glomerular hyperfiltration in early diabetes. |
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