| 王芳宇.噬菌体随机肽库在确定单抗识别表位中的应用.[J].中南医学科学杂志.,2001,(1):1-3,5. |
| 噬菌体随机肽库在确定单抗识别表位中的应用 |
| Identification of Epitope Recognized by Monoclonal Antibody with Phage-displayed Random Peptide Library |
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| DOI： |
| 中文关键词: 噬菌体展示 多肽 单抗 亲和筛选 表位 |
| 英文关键词:phage display,peptide,monoclonal antibody,affinity screening,epitode |
| 基金项目: |
| 王芳宇 |
| 南华大学生物化学教研室!湖南衡阳421001 |
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| 中文摘要: |
| 以抗人感冒病毒凝血素蛋白单克隆抗体12CA5为筛选配基，从丝状噬菌体（M13）表面展示的随机6肽库中筛选抗体特异性识别的多肽，噬菌体与单抗的结合可以用酶联免疫吸附法鉴定，而展示的氨基酸序列可以通过测定相应克隆的插入外源核苷酸来确定，筛选所犁多肽与单抗的表达，特别是C-蝗四个氨基酸残基PDYA有高度的同源性，说明该四肽在单抗与抗原的结合中起到十分重要的作用。 |
| 英文摘要: |
| Objective: To select and identify the peptides specifically recognizing the monoclonal antibody (mAb) 12CA5 against human influenza hemagglutinin from a hexapeptide library dispalied on the surface of filamentous bacteriophage(M13). Methods: The binding of phage to mAb was detected by the enzyme-linked immunosorbent assays, while the amino acid sequences of displayed peptides was confirmed by sequencing the inserted nucleotide acid sequences of corresponding clones.Results: The amino acid sequences of obtained peptides show high homology to the epitope of mAb 12CA5, especially to the four residues PDYA at the C-terminus.Conclusion:quarta peptide PDYA plalys an important role in the binding of antigen to mAb. |
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