| 龙鼎新,吴成秋,陈雯.大鼠正常组织中的端粒酶活性及意义.[J].中南医学科学杂志.,2000,(1):. |
| 大鼠正常组织中的端粒酶活性及意义 |
| Significance of Telomerase Activity in Normal Tissues in Rats |
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| DOI： |
| 中文关键词: 端粒酶,TRAP,定量检测,液体闪烁计数,大鼠 |
| 英文关键词:WT5BZ] telomerase,TRAP,quantitative measurement,scintillation count assay,rat |
| 基金项目: |
| 龙鼎新 吴成秋 陈雯 |
| 衡阳医学院卫生学教研室!湖南衡阳421001 |
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| 中文摘要: |
| 为探讨大鼠正常组织中的端粒酶活性及意义 ,采用TRAP结合液闪计数法定量测定了成年SD大鼠大肠粘膜、肺脏、肝脏、睾丸及附睾组织中的端粒酶活性。结果显示 :这些组织中都有不同程度的端粒酶活性 ,其中睾丸组织中端粒酶活性水平最高。说明大鼠正常组织中有不同程度的端粒酶活性表达 ,并可能与人类正常组织中的调节机制不同。 |
| 英文摘要: |
| To study the significance of telomerase activity in normal tissues in rats,the method of combining telomeric repeat amplification protocol (TRAP) and scintillation count assay was used to quantify telomerase activity from colon mucosa, lung, liver, testicle and epididymis in Sprague-Dawley rats.The results showed that all of these tissues had telomerase activity,and the level of telomerase activity in testicle was the highest. It suggests that the telomerase activity is various in different tissues in rats, and that the telomerase regulation mechanism in rats maybe differ from that in human. [WT5HZ] |
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