张,曾明新,徐惠芳.检测幽门螺杆菌感染几种方法的比较.[J].中南医学科学杂志.,1994,(1):.
检测幽门螺杆菌感染几种方法的比较
Comparison of Several Methods for the Detection of Helicobacter Pylori Infection
  
DOI:
中文关键词:  幽门螺杆菌,检测方法,比较
英文关键词:Helicobacter pylori,Detection mothods,Comparison.,
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  曾明新  徐惠芳
Department of Internal Medicine,Second Affliated Hospital,Hengyang Medical College
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中文摘要:
      本文对95例因上消化道症状行内镜检查患者的胃粘膜组织进行幽门螺杆菌检查。细菌培养、尿素酶试验、直接涂片、HE染色、Giemsa染色及改良Warthin-Starry银染检出率分别为63.2%、69.5%、60.1%、58.9%、71.6%、74.7%。结果表明改良Warthin-Starry银染检出率最高,HE染色最低,二者比较差异有显著性(P<0.05),尿素酶试验及Giemsa染敏感性及特异性均高,尿素酶试验结合直接涂片法有简单、快速、经济、敏感性及特异性高之优点。
英文摘要:
      In this paper,the detection of helicobacter pylori infection was performed on gastric mucosa obstained from biopsies of 95 patients undergoing endscopy for various upper gastrointestinal diseases.Helicobacter pylori infection was detected by methods including bacteria culture,urease test,direct smear,hematoxylin and eosin staining,Giemsa staining and modified Warthin-Starry staining,The positive valuse of the above methods was 63.2%,69.5%,60.1%,58.9%,71.6%,74.7%,respectively.The results showed that the modified Warthin-Starry stain was the most sensitive among the above methods,while hematoxylin and eossin staining showed the lowest positive value (P<0.05).Sensitivity and specificity of the urease test and Giemsa staining was the higher (P>0.05).Urease test combined with direct smear has an advatage in ease,lower cost,rapidity,sensitivity and sepcificity for the detection of Helicobacter pylori.
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