邓健,樊华.苯酚—硫酸法测定茯苓中多糖含量.[J].中南医学科学杂志.,1993,(3):. |
苯酚—硫酸法测定茯苓中多糖含量 |
Phenol-Sulfuric Acid Method for Determining Polysaccharide Content in Poria cocos Wolf |
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DOI: |
中文关键词: 茯苓,多糖,多糖含量测定,苯酚—硫酸法 |
英文关键词:Poria cocos Wolf,polysaccharide,phenol-sulfuric acid method |
基金项目: |
邓健 樊华 |
衡阳医学院化学教研室,中南工学院 |
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中文摘要: |
用尿素溶液作为天然水不溶性多糖的提取溶剂,以苯酚—浓硫酸作显色剂,在波长490nm下对七个不同产地的茯苓样品进行了多糖的含量测定,其含量范围为81.9%~88.3%。该法操作简便,结果准确。 |
英文摘要: |
Polysaccharides in Poria cocos Wolf from different localities were extracted with urea solution and were determined by spectrophotometry at 490nm with phenol-concentrated sulfuric acid as the color developer. The content of polysaccharides in seven samples ranged from 81.9% to 88.3%. The method is simple and accurate for the quntitative determination of natural water insoluble polysaccharides. |
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