| 彭宣宪,陈富生,王三英.应用捕捉法ELISA检测乙肝患者血清中IgG类HBsAg特异性免疫复合物.[J].中南医学科学杂志.,1990,(1):. |
| 应用捕捉法ELISA检测乙肝患者血清中IgG类HBsAg特异性免疫复合物 |
| DETECTION OF HBsAg SPECIFIC CIRCULATING IMMUNE-COMPLEXES OF IgG IN HEPATITISB BY ACCA-ELISA |
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| DOI： |
| 中文关键词: 酶联免疫吸附测定,乙型病毒性肝炎,免疫球蛋白G,循环免疫复合物 |
| 英文关键词:ELISA,hepatitis B,IgG,circulating immune complexes |
| 基金项目: |
| 彭宣宪 陈富生 王三英 |
| 江西大学生物系微生物学研究室，衡阳医学院生物化学教研室，江西省安福县人民医院 |
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| 中文摘要: |
| 本文建立了一种检测HBsAg/IgC—CIC(Circulating ImmuneComplex)捕捉法ELISA(Enzyme Linked Immunosorbert Assays)。该法特异性强,敏感性高,重复性好,避免了固相抗HBs双抗体ELISA受血清中游离HBsAg的抑制作用而影响HBsAg/IgG—CIC检出的缺点。并行检测HBsAg/IgG—CIC和血清C_3发现,乙肝患者血清中HBsAg/IgG—CIC检出率血清C_3下降显著高于非下降组,提示HBsAg/IgG—CIC可能是导致乙肝患者血清C_3下降的又一重要因素。 |
| 英文摘要: |
| An assay for the specific detection of HBsAg/IgG-CIC by ACCA-ELISA (antibody class capture assay-ELISA) was reported in this paper. This method is not only simple, specific, sensitive and reproducible, but also free of the weakness of inhibiting detection of HBsAg/IgG-CIC by serum HBsAg which existed in the ELISA using anti-HBs attached on solid phase. The result that both C3 and HBsAg/IgG-CIC were detected in one serum sample indicates that positive rates of specific HBsAg/IgG-CIC in the patients with low level of serum C3 were signifcantly higher than in those with normal level. It suggests that HBsAg/IgG-CIC be one of important substances that make serum C3 reduced decline. |
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